Part:BBa_K404300

SEG-Linker

Amino acid sequence:
GGSGGGSEGGGSEGGGSEGGGSEGGGSEGGGSGGGS

This part is a peptide linker, it can be used to connect two protein parts / domains and add additional space between them. This can be necessary to avoid interactions between the different parts when fusion proteins are created. The SEG linker was created to connect our protein domains to a fusion construct.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

Parameters for BioBrick BBa_K404300 automatically created by the BioBrick-AutoAnnotator version 1.0
Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein)  ATGGCCGGCGGTGGTTCT ... GGCGGTAGCACCGGT  ORF from -8 to 114 (excluding stop-codon)
Amino acid sequence: (RFC25 scars in shown in bold, other sequence features underlined; both given below) 1  MAGGGSGGGSEGGGSEGGGSEGGGSEGGGSEGGGSGGGSTG*
Sequence features: (with their position in the amino acid sequence, also underlined or shown bold above)
Amino acid composition: A (Ala) 1 (2.4%) R (Arg) 0 (0.0%) N (Asn) 0 (0.0%) D (Asp) 0 (0.0%) C (Cys) 0 (0.0%) Q (Gln) 0 (0.0%) E (Glu) 5 (12.2%) G (Gly) 25 (61.0%) H (His) 0 (0.0%) I (Ile) 0 (0.0%) L (Leu) 0 (0.0%) K (Lys) 0 (0.0%) M (Met) 1 (2.4%) F (Phe) 0 (0.0%) P (Pro) 0 (0.0%) S (Ser) 8 (19.5%) T (Thr) 1 (2.4%) W (Trp) 0 (0.0%) Y (Tyr) 0 (0.0%) V (Val) 0 (0.0%)
Amino acid counting Total number: 41 Positively charged (Arg+Lys): 0 (0.0%) Negatively charged (Asp+Glu): 5 (12.2%)	Biochemical parameters Molecular mass [Da]: 3089.9 Theoretical pI: 3.51 Extinction coefficient at 280 nm [M-1 cm-1]: 0 / 0 (all Cys red/ox)
Codon usage Organism: E. coli B. subtilis S. cerevisiae A. thaliana Mammals Codon quality (CAI): excellent (0.89) good (0.75) good (0.63) good (0.61) good (0.80)
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation. If you have any questions, comments or suggestions, please email us at igem@wzw.tum.de.